October 28th CTE Seminar

Student Presentation 1

Title: Conformal 3D Printing on Objects with Near-Arbitrary Organic Shape using
3D Scanning and Point Cloud Data

Presenting author: Ezgi Kucukdeger, Industrial Systems Engineering

Research group: Advanced Biomanufacturing and Biosensing Laboratory

Additive manufacturing, commonly referred to as 3D printing, has several advantages over thetraditional manufacturing processes. Directing computer-driven robotics not only enables controlover the parts spatial structure and composition, and thus, the parts material properties but alsofacilitates novel applications in conformal manufacturing. However, the current state of the artfor conformal additive manufacturing can only be used to: 1) 3D print on objects whose shapeis described by analytical functions, and 2) 3D print tool paths that were significantly smallerthan the objects radius of curvature. The purpose of this work was to create a methodology thatenables conformal 3D printing on objects with near arbitrary organic shape using tool paths thathave a comparable length scale to the objects radius of curvature. A set of conformal printingalgorithms incorporating structured light 3D scanning as an imaging technique was provided forquality conformal 3D printing of pre-defined linear and circular tool paths. New insights intothe relationship between surface topographical parameters and nozzle-surface offset are provided.Novel algorithms for conformal 3D printing are potentially useful to a wide range of fields andindustries, including the healthcare, electronics, and aerospace industries, in the form of in situbioprinting, biomedical device manufacturing, and part certification applications, respectively.

Student Presentation 2

Title: Spatiotemporal variation in functional markers along the rat jejunum and its effect on hepatocytes in an in vitro gut-liver model

Presenting author: Anjaney Kothari, Biomedical Engineering and Sciences

Research group: Laboratory for Biomaterials and Tissue Engineering

In vivo studies have established the occurrence of several location-dependent trends along thelength of the small intestine from duodenum to jejunum to ileum. However, the length scale ofmanifestation of functional and morphological markers of the intestine has not been investigatedbefore. In particular, in vitro studies on location-dependent variation in the intestine are absentfrom literature. The first part of this talk would discuss the use of rat intestinal explants isolatedand cultured in a novel manner to understand spatiotemporal differences along the rat jejunum inculture. Spatial trends were assessed across three regions of the jejunum: proximal, medial anddistal. Different cell types of the intestinal epithelium showed a temporal dependence of gradationin functional markers in vitro. While enterocyte function showed gradation in properties as earlyas 4 h in culture, lysozyme and goblet cell functions showed a significant spatial dependence at 24h. The second part of the talk would focus on integration of jejunum explants with hepatocytes ina collagen sandwich model to study the two organs holistically. Notable beneficial effects of inte-gration were observed on both the organs in urea production by hepatocytes and mucin productionby the jejunum explants. This study demonstrates spatial and temporal sensitivity in gradation ofdifferent markers along the intestine in vitro. Further, the evaluation of integrated models of the intestine and liver hints at their potential for future studies on the gut-liver axis.